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Aptamer conjugated Mo6S9−xIx nanowires for direct and highly sensitive electrochemical sensing of thrombin

McMullan, M, Sun, N, Papakonstantinou, P, Li, MX, Zhou, W and Mihalilovic, D (2011) Aptamer conjugated Mo6S9−xIx nanowires for direct and highly sensitive electrochemical sensing of thrombin. Biosensors and Bioelectronics, 26 . pp. 1853-1859. [Journal article]

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DOI: doi:10.1016/j.bios.2010.01.030


We demonstrate the use of a novel electrochemical sensing platform based on aptamer conjugated Mo6S9−xIx nanowires (MoSI NWs) for the highly sensitive detection of the blood clotting enzyme thrombin.MoSI NWs nanowires were self-assembled on a gold electrode to which thrombin binding aptamers were covalently attached. Themodification and immobilization steps of the electrodeswere characterised by cyclic voltammetry along with high-resolution transmission electron microscopy and X-ray photoelectron spectroscopy. The platform is based on the creation of a self-assembled MoSI MWlayer via thesulfur–gold affinity followed by the creation of MoSI-thiolated aptamer conjugates via the sulfur–sulfur affinity. Using this system, sensitive quantitative detection of thrombin is realized by monitoring differences of differential pulse voltammetric responses of electrostatically trapped [Ru(NH3)6]3+ cations to the aptamer before and after thrombin binding. The sensitivity limit for the detection of thrombin is 10 pM. This value is 10-fold better than all currently reported one step label free electrochemical strategies. Given the direct label free nature of the approach and the simplicity of the electronic detection, theaptamer conjugated MoSI NWs biosensor appears well suited for implementation in portable point of care microdevices directed at the rapid and sensitive detection of proteins and pathogens.

Item Type:Journal article
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:14220
Deposited By: Professor Pagona Papakonstantinou
Deposited On:14 Jun 2010 09:47
Last Modified:30 Sep 2013 14:31

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