Nekrassova, O, White, PC, Threlfell, S, Hignett, G, Wain, AJ, Lawrence, NS, Davis, J and Compton, RG (2002) An electrochemical adaptation of Ellman's test. ANALYST, 127 (6). pp. 797-802. [Journal article]
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The electrochemically initiated reaction of thiols with N,N-diethyl-p-phenylenediamine has been coupled with an existing colorimetric sensing reaction developed by Ellman as a means of providing an electrochemical adaptation of the latter whereby the total thiol species present in a sample can be determined. The detection methodology has been proven to be robust with a linear range for cysteine from 2-120 muM, a limit of detection of 1.17 muM and has shown selectivity against a wide range of potential interferences. The efficiency of the methodology has been examined in the determination and recovery of thiol species in three growth tissue media, which contain a number of common biological interferences.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Computing & Engineering|
Faculty of Computing & Engineering > School of Engineering
|Research Institutes and Groups:||Engineering Research Institute|
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
|Deposited By:||Professor James Davis|
|Deposited On:||01 Jul 2010 08:33|
|Last Modified:||18 Aug 2011 10:19|
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