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Correlation between microbial protein thermostability and resistance to denaturation in aqueous-organic solvent 2-phase systems

Owusu, RK and Cowan, DA (1989) Correlation between microbial protein thermostability and resistance to denaturation in aqueous-organic solvent 2-phase systems. ENZYME AND MICROBIAL TECHNOLOGY, 11 (9). pp. 568-574. [Journal article]

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URL: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG1-47DM40H-CN&_user=126978&_coverDate=09%2F30%2F1989&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1413093069&_rerunOrigin=google&_acct=C000010438&_version=1&_urlVersion=

DOI: doi:10.1016/0141-0229(89)90084-7


The thermostability of cell-free protein extracts (from 10 microorganisms grown at 37–84°C) and of 10 fully or partially purified neutral proteases was determined in aqueous solution (Tris-HCl buffer 0.1 m. pH 7.0) using protein solubility and enzyme activity loss, respectively, as the criteria of denaturation. The stability of the protein extracts and purified proteins was also determined in an aqueous-water immiscible organic solvent two-phase system using the same criteria. There was a strong positive correlation between microorganism growth temperature and the thermostability of protein extracts in both aqueous and aqueous:organic two-phase systems. A correlation also existed between protease thermostability and the stability in the aqueous:organic two-phase system. Protein stability was higher in those aqueous:organic two-phase systems with a more hydrophobic organic phase. However, for pairs of organic solvents of similar hydrophobicity, the aqueous:organic two-phase system with the higher rated interfacial tension was more denaturing. Protein extract stability was sensitive to both aqueous:gas and aqueous:organic interface size depending on the nature of the organic phase.

Item Type:Journal article
Keywords:Thermophilic proteins; protein thermostability: protein stability; aqueous:organic solvent two-phase systems
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
ID Code:14682
Deposited By: Dr Richard Owusu-Apenten
Deposited On:03 Aug 2010 08:01
Last Modified:28 Jan 2014 14:46

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