Osborne, NN, FitzGibbon, F and Schwartz, G (1991) Muscarinic Acetylcholine Receptor-Mediated Phosphoinositide Turnover in Cultured Human Retinal Pigment Epithelium Cells. Vision Research, 31 (7-8). pp. 1119-1127. [Journal article]
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Cultured retinal pigment epithelium cells prepared from post-mortem adult human eyes are shown to contain muscarinic receptors associated with phosphoinositide turnover. Carbachol at a concentration of 100μM induced a four-fold increase in3H-inositol phosphates (more than 76% is in the form of3H-inositol-1-phosphate) accumulation within 45 min in cells prelabelled with3H-myoinositol and exposed to 5mM LiCl. The EC50 of carbachol was approx. 70μM and the saturation concentration was about 1 mM. The carbachol-induced response was blocked by both atropine and pirenzepine, the former being most effective. Pre-exposure of cells to carbachol resulted in desensitization and a drastic reduction in the subsequent carbachol-induced stimulation of3H-inositol phosphates. The carbachol response could be attenuated by the biologically active phorbol ester, 4β-phorbol 12-myristate 13-acetate, and this was nullified by the protein kinase C inhibitor, staurosporine. The biologically inactive phorbol ester, 4α-phorbol 12,13 dideconoate, did not attenuate the carbachol-induced stimulation of3H-inositol phosphates. Pertussis toxin failed to influence the carbachol receptor-mediated phosphoinositide turnover. These studies provide clear evidence for the occurrence of muscarinic receptors coupled to phosphoinositide hydrolysis on human retinal pigment epithelium cells.
|Item Type:||Journal article|
|Keywords:||Phosphoinositide turnover;pigment epithelium cells;cholinergic receptors|
|Faculties and Schools:||Faculty of Computing & Engineering|
Faculty of Computing & Engineering > School of Engineering
|Research Institutes and Groups:||Engineering Research Institute|
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
|Deposited By:||Mr Francis FitzGibbon|
|Deposited On:||16 Sep 2010 13:46|
|Last Modified:||04 Oct 2010 11:22|
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