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Epidermal Growth factor potentiates the Transmitter-Induced Stimulation of C-AMP and Inositol Phosphates in Human Pigment Epithelial Cells in Culture

Liu, NP, FitzGibbon, F, Nash, M and Osborne, NN (1992) Epidermal Growth factor potentiates the Transmitter-Induced Stimulation of C-AMP and Inositol Phosphates in Human Pigment Epithelial Cells in Culture. Experimental Eye Research, 55 (3). pp. 489-497. [Journal article]

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URL: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WFD-4BJVWTP-V6&_user=4008657&_coverDate=09%2F30%2F1992&_rdoc=14&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%236792%231992%23999449996%23477109%23FLP%23display%

DOI: 10.1016/0014-4835(92)90122-9


Salbutamol, isoproterenol and dopamine stimulate C-AMP production in human retinal pigment epithelium (RPE) cells by activation of β2-type receptors. Epidermal growth factor (EGF) in contrast does not alter basal levels of C-AMP but elevates in an apparently dose-dependent manner the isoproterenol-induced stimulation of C-AMP. EGF also potentiates the forskolin-induced stimulation of C-AMP but has no effect on the elevation of C-AMP caused by NECA (5′-[N-ethyl]-carboxamido adenosine), an adenosine A2-receptor agonist. EGF, isoproterenol and NECA have no effect on basal levels of inositol phosphates (InsPs) in human RPE cells, but EGF specifically elevates the carbachol-induced stimulation of InsPs. The carbachol effect on InsPs is attenuated by the phorbol ester PMA (4β-phorbol 12 myrisate 13-acetate). PMA did not, however, affect the stimulation of C-AMP caused by isoproterenol. The interaction of EGF and C-AMP is further demonstrated in experiments where the incorporation of [3H]thymidine into RPE cells was studied, as an index for proliferation. EGF stimulates RPE cell proliferation while isoproterenol and dibutyryl C-AMP nullify the EGF effect. Dibutyryl C-AMP has a negative effect on RPE cell proliferation while isoproterenol is ineffective.The data presented here suggest that after stimulation of EGF receptors, tyrosine-kinase-activated products can influence secondary messenger products produced from activation of β2-type (linked with C-AMP formation) and muscarinic (linked with InsPs production) receptors in RPE cells. We could find no evidence of an interaction between receptors associated with C-AMP and InsPs/diacylglycerol production.

Item Type:Journal article
Keywords:Epidermal growth factor;C-AMP;inositol phosphates;pigment epithelial cells
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:15631
Deposited By: Mr Francis FitzGibbon
Deposited On:16 Sep 2010 13:45
Last Modified:04 Oct 2010 11:22

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