Chen, TB, Farragher, S, Bjourson, AJ, Orr, DF, Rao, P and Shaw, C (2003) Granular gland transcriptomes in stimulated amphibian skin secretions. BIOCHEMICAL JOURNAL, 371 (Part 1). pp. 125-130. [Journal article]
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Amphibian defensive skin secretions are complex, species-specific cocktails of biologically active molecules, including many uncharacterized peptides. The study of such secretions for novel peptide discovery is time-limited, as amphibians are in rapid global decline. While secretion proteome analysis is non-lethal, transcriptome analysis has until now required killing of specimens prior to skin dissection for cDNA library construction. Here we present the discovery that polyadenylated mRNAs encoding dermal granular gland peptides are present in defensive skin secretions, stabilized by endogenous nucleic acid-binding amphipathic peptides. Thus parallel secretory proteome and transcriptome analyses can be performed without killing the specimen in this model amphibian system - a finding that has important implications in conservation of biodiversity within this threatened vertebrate taxon and whose mechanistics may have broader implications in biomolecular science.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences|
Faculty of Life and Health Sciences > School of Biomedical Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Biomedical Sciences Research Institute > Stratified Medicine
|Deposited By:||Mrs Caroline Adams|
|Deposited On:||30 Nov 2009 09:37|
|Last Modified:||14 Oct 2013 14:29|
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