Rollan, A, McCormack, D, McCormack, H, McHale, L and McHale, AP (1996) A rapid in situ, colorimetric assay for the determination of mammalian cell viability in alginate-immobilized and encapsulated systems. BIOPROCESS ENGINEERING, 15 (1). pp. 47-49. [Journal article]
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A convenient, rapid colorimetric assay system has been developed in order to determine cell viability of populations of mammalian cells encapsulated using a poly-L-lysine/alginate encapsulation system. The method is based on the use of 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and involves direct incubation of the capsules with the reagent. This is followed by direct solubilization of the resulting formazan product in dimethyl sulfoxide (DMSO). In order to demonstrate that this assay method may be generally applicable to alginate-based carrier systems, viability of cells immobilized in alginate beads, as distinct from encapsulation in the poly-L-lysine/alginate system, was also determined using the in situ method.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science|
Faculty of Life and Health Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
|Deposited By:||Professor Anthony McHale|
|Deposited On:||28 Mar 2012 15:21|
|Last Modified:||04 Dec 2012 11:51|
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