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Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn

Smyth, Franklin, Smyth, T. J. P., Ramachandran, V. N., O'Donnell, F. and Brooks, P. (2012) Dereplication of phytochemicals in plants by LC-ESI-MS and ESI-MSn. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 33 . pp. 46-54. [Journal article]

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DOI: 10.1016/j.trac.2011.09.015


This review discusses how crude extracts of selected plants are subjected to column chromatography and the resulting fractions tested for their antibacterial activity towards organisms (e.g., methicillin-resistant-Staphylococcus aureus). We discuss the bioactive column-chromatography fractions being further separated by preparative thin-layer chromatography and the resulting bands being investigated by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (LC-ESI-MS) and ESI-MSn. We then show how the resulting retention times, molecular masses, their fragmentation patterns, the Chemnet database and an in-house quinoline database are used in the dereplication process by structural elucidation of some of the compounds when compared with known structures of natural origin. Some molecular masses and the corresponding fragmentations do not correlate with any known compounds, thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multi-drug-resistant microorganisms. (C) 2012 Elsevier Ltd. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:22064
Deposited By: Prof W.Franklin Smyth
Deposited On:08 May 2012 10:56
Last Modified:08 May 2012 10:56

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