Barnes, Clifford, O'Hagan, Barry, Howard, Vyvyan and McKerr, G (2007) Verification of cell viability at progressively higher scanning forces using a hybrid atomic force and fluorescence microscope. Journal of microscopy, 228 (Pt 2). pp. 185-9. [Journal article]
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The prudent use of the atomic force microscope as a supra-vital live cell imaging tool requires that cell viability must be determined before and after scanning. Complementary optical techniques in conjunction with the fluorescent dyes rhodamine-123 and ethidium homodimer have been used within this study to determine cell viability after increasing loads are applied in contact mode. Guideline force ranges for five commonly cultured cell lines, human squamous carcinoma (A431), fibroblast, HeLa, Potorous tridactylis (PtK2) and rat intestinal epithelial (RIE) cells are given.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences > School of Biomedical Sciences|
Faculty of Life and Health Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute > Molecular Medicine|
Biomedical Sciences Research Institute
|Deposited By:||Dr Barry O'Hagan|
|Deposited On:||15 May 2012 10:49|
|Last Modified:||15 May 2012 10:49|
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