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In vitro fermentation and prebiotic potential of novel low molecular weight polysaccharides derived from agar and alginate seaweeds

Ramnani, Priya, Chitarrari, Roberto, Tuohy, Kieran, Grant, John, Hotchkiss, Sarah, Philp, Kevin, Campbell, Ross, Gill, Chris and Rowland, Ian (2012) In vitro fermentation and prebiotic potential of novel low molecular weight polysaccharides derived from agar and alginate seaweeds. Anaerobe, 18 (1). p. 1. [Journal article]

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URL: http://dx.doi.org/10.1016/j.anaerobe.2011.08.003

DOI: doi:10.1016/j.anaerobe.2011.08.003

Abstract

Fermentation properties and prebiotic potential of novel low molecular weight polysaccharides (LMWPs)derived from agar and alginate bearing seaweeds was investigated. Ten LMWPs were supplemented to pH, temperature controlled anaerobic batch cultures inoculated with human feces from three donors, in triplicate. Microbiota changes were monitored using Fluorescent in-situ hybridization and short chain fatty acids, the fermentation end products were analysed using gas chromatography. Of the ten LMWPs tested, Gelidium seaweed CC2253 of molecular weight 64.64 KDa showed a significant increase in bifidobacterial populations from log10 8.06 at 0 h to log10 8.55 at 24 h (p ¼ 0.018). For total bacterial populations, alginate powder CC2238 produced a significant increase from log10 9.01 at 0 h to log10 9.58 at 24 h (p ¼ 0.032). No changes were observed in the other bacterial groups tested viz. Bacteroides, Lactobacilli/ Enterococci, Eubacterium rectale/Clostridium coccoides and Clostridium histolyticum. The polysaccharides also showed significant increases in total SCFA production, particularly acetic and propionic acids, indicating that they were readily fermented. In conclusion, some LMWPs derived from agar and alginate bearing seaweeds were fermented by gut bacteria and exhibited potential to be used a novel source of prebiotics.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
ID Code:22128
Deposited By: Dr Chris Gill
Deposited On:22 Jan 2014 09:55
Last Modified:09 May 2016 11:11

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