Millar, C, Moore, J, Lowery, C, McCorry, K and Dooley, J (2001) Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple methods suited for outbreak analysis. INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, 204 (2-3). pp. 191-194. [Journal article]
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A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitives inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as Such was a suitable template for the detection of C. parvum by various PCR amplification targets.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences > School of Biomedical Sciences|
Faculty of Life and Health Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)|
Biomedical Sciences Research Institute
|Deposited By:||Professor James Dooley|
|Deposited On:||15 May 2012 10:53|
|Last Modified:||17 May 2012 13:22|
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