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Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple methods suited for outbreak analysis

Millar, C, Moore, J, Lowery, C, McCorry, K and Dooley, J (2001) Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple methods suited for outbreak analysis. INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, 204 (2-3). pp. 191-194. [Journal article]

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DOI: 10.1078/1438-4639-00090

Abstract

A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitives inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as Such was a suitable template for the detection of C. parvum by various PCR amplification targets.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
ID Code:22232
Deposited By: Professor James Dooley
Deposited On:15 May 2012 10:53
Last Modified:17 May 2012 13:22

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