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TEMPERATURE-DEPENDENT PROTEIN AND LIPOPOLYSACCHARIDE EXPRESSION IN CLINICAL AEROMONAS ISOLATES

Tso, MD and Dooley, James (1995) TEMPERATURE-DEPENDENT PROTEIN AND LIPOPOLYSACCHARIDE EXPRESSION IN CLINICAL AEROMONAS ISOLATES. JOURNAL OF MEDICAL MICROBIOLOGY, 42 (1). pp. 32-38. [Journal article]

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Abstract

Clinical isolates of Aeromonas were grown at eight different temperatures from 10 degrees C to 40 degrees C. Whole cell lysates were examined by SDS-PAGE and major temperature-dependent changes to both protein and lipopolysaccharide (LPS) profiles were identified. Cells grown at the higher temperatures (37 degrees C and 40 degrees C) produced abundantly a protein of c. 60 kDa which was not detected at the lower temperatures. Temperature-dependent expressions of other proteins were also noted but these were more variable among the isolates. An effect of temperature on expression of lipopolysaccharides was also noted in that some strains produced significantly less O-polysaccharides at the higher temperatures. After fractionation of cells, major differences in the expression of cell envelope and outer-membrane proteins between cells grown at low and high temperatures were noted although no unifying patterns could be discerned. Such growth temperature-induced changes in the cell envelope constituents have not been described previously for Aeromonas isolates from man.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
ID Code:22238
Deposited By: Professor James Dooley
Deposited On:15 May 2012 12:01
Last Modified:17 May 2012 13:29

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