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Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis

Gibson, David, Newell, Keri, Evans, Alexandra, Finnegan, Sorcha, Manning, Gwen, Scaife, Caitiona, McAllister, Catherine, Pennington, Stephen, Duncan, Mark, Moore, Terry and Rooney, Madeleine (2012) Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis. Journal of Proteomics, 75 . pp. 5479-5492. [Journal article]

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DOI: 10.1016/j.jprot.2012.06.024


Introduction. Juvenile idiopathic arthritis (JIA) comprises a poorly understood group ofchronic autoimmune diseases with variable clinical outcomes. We investigated whetherthe synovial fluid (SF) proteome could distinguish a subset of patients in whom diseaseextends to affect a large number of joints.Methods. SF samples from 57 patients were obtained around time of initial diagnosis of JIA,labeled with Cy dyes and separated by two-dimensional electrophoresis. Multivariateanalyses were used to isolate a panel of proteins which distinguish patient subgroups.Proteins were identified using MALDI-TOF mass spectrometry with expression verified byimmunochemical methods. Protein glycosylation status was confirmed by hydrophilicinteraction liquid chromatography.Results. A truncated isoform of vitamin D binding protein (VDBP) is present at significantlyreduced levels in the SF of oligoarticular patients at risk of disease extension, relative toother subgroups (p<0.05). Furthermore, sialylated forms of immunopurified synovial VDBPwere significantly reduced in extended oligoarticular patients (p<0.005).Conclusion. Reduced conversion of VDBP to a macrophage activation factor may be used tostratify patients to determine risk of disease extension in JIA patients.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:23513
Deposited By: Dr David Gibson
Deposited On:08 Oct 2012 08:40
Last Modified:07 Apr 2014 08:53

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