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Antibiotic treatment of exacerbation of chronic airways infection

Gibson, David (2012) Antibiotic treatment of exacerbation of chronic airways infection. In: 35th European Cystic Fibrosis Conference, Dublin, Ireland. Elsevier B.V.. 1 pp. [Conference contribution]

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Abstract

Objectives: Repeated cycles of pulmonary exacerbation of chronic infection causetissue damage and progressive drop in lung function for CF patients so makingeffective treatment and prevention strategies imperative. Our study aims to betterunderstand the response to antibiotics and identify biomarker targets suitable forimproved therapeutic or preventative treatments.Methods: Multidimensional LC-MS/MS and relative quantitation was employed toinvestigate the changes in sputum cellular proteome following antibiotic treatmentin 12 CF patients chronically infected with Pseudomonas aeruginosa.Conclusions: 1989 human proteins were identified in total. 37 proteins out ofthe 318 detected in >80% samples were differentially expressed (p<0.05) afterantibiotic treatment. Of these, 22 exhibited a strong correlation (r = 0.969) betweentheir pre-antibiotic/post-antibiotic ratios and ratios for the same proteinscomparing CF/healthy control cohorts, thus suggesting that successful treatmentpromotes a proteome shift towards a non-CF profile. Ingenuity Pathway Analysissoftware showed antibiotic treatment to most affect the molecular and cellularfunction categories of Cellular Movement (particularly Immune Cell Trafficking),Organismal Injury and Abnormalities, Cell Death and Free Radical Scavenging.67 P. aeruginosa proteins and 17 proteins from other bacterial species werealso detected. Bacterial proteins accounted for 0.5−35% of CF sputum cellularprotein. Although total cellular protein (per g sputum) decreased following antibiotictreatment, the percentage attributable to bacteria did not alter and no bacterialproteins were detected as differentially expressed.

Item Type:Conference contribution (Poster)
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:24359
Deposited By: Dr David Gibson
Deposited On:07 Jan 2013 10:03
Last Modified:09 Dec 2015 11:09

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