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Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry.

Hughes, RK, Wu, Z, Hornostaj, AR, Robinson, DS, Hardy, D, West, SI, Owusu-Apenten, Richard, Fairhurst, SA, Chan, M, Lawson, DM and Casey, R (1999) Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry. Agro Food Industry Hi-Tech, 10 (3). pp. 3-6. [Journal article]

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Abstract

Following an introduction to application of lipoxygenases (LOX) from peas and potatoes in the agrifood industry, this paper discusses: properties of recombinant LOX prepared from pea seeds and potato tubers; probing the primary determinants of LOX-catalysis; and applications of recombinant LOX. 3 novel recombinant LOX have been expressed in Escherichiacoli, purified and characterized. These have distinct properties and reaction mechanisms for probing primary determinants of LOX-catalysis and for agrifood applications. Studies on pea LOX-3 have provided new insights into cooxidation chemistry. Production of mutant LOX and analysis of LOX sequences in relation to homology models gives a key to understanding primary determinants of LOX-catalysis. This further extends the range of recombinant LOX available and should allow production of customized biocatalysts for industry.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
ID Code:24436
Deposited By: Dr Richard Owusu-Apenten
Deposited On:09 Jan 2013 11:12
Last Modified:13 Aug 2015 13:47

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