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Gibson, David (2008) SYNOVIAL FLUID PROTEOME EXPRESSION PATTERNSSEGREGATE JUVENILE IDIOPATHIC ARTHRITIS PATIENTS. In: British Society for Rheumatology, Liverpool. Oxford Journals. Vol 47 (Suppl) 1 pp. [Conference contribution]

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Background: Synovial fluid (SF) is a potential source of novel biomarkers formany arthritic disorders involving joint inflammation, including Juvenile IdiopathicArthritis (JIA). We first compared the distinctive protein expression patterns of localjoint inflammation in SF with systemic profiles within matched plasma samples.Preliminary investigations were performed into whether local or systemic proteome‘fingerprints’ could distinguish between oligoarticular, extended oligoarticular andpolyarticular forms of this chronic juvenile disease.Methods: In this study we analysed matched SF and plasma samples obtainedfrom 10 newly diagnosed JIA patients (<6 months disease duration): 3 with oligoarticulararthritis, 3 extended oligoarticular and 4 polyarticular disease. Matchedsamples were taken at the initial inflammatory episode. We profiled the SF andplasma proteomes using a two-dimensional difference gel electrophoresis (DIGE)approach. Progenesis PG240 software analysis of plasma and SF gel scans wasused to highlight joint-specific and plasma proteins differentially expressed acrossthe study group. Protein spots of interest were identified by matrix-assisted laserdesorption ionization (MALDI-TOF) and confirmed by nanoelectrospray-ionisationmass spectrometry.Results: 2D DIGE reveals 899 spots per gel within the pH 4–7 range for synovialfluid and plasma. Comparison of plasma and synovial gel scans, revealed a subpopulationof 143 spots which predominate in synovial fluid or plasma. Hierarchicalclustering based on the expression levels of a set of 54 proteins with at least twofold expression differences between the two body fluids segregates the synovialfluid from the plasma samples. Proteolytic fragments of anti-inflammatory proteinsinter-alpha trypsin inhibitor, alpha-1 antitrypsin, transthyretin and apolipoprotein A-1were identified. Principle component analysis of five different protein features couldbe used to segregate patients into clinical subgroups.Conclusions: Synovial fluid and plasma proteomes can be used to segregate aheterogeneous group of JIA patients into clinical subgroups. Such an approach couldallow us to identify biomarkers useful in the prediction of disease progression, andtherefore enable earlier and more appropriate therapeutic intervention. Definition ofprotein profiles which discriminate clinical subgroups of arthritic disease may assistin the diagnosis of juvenile arthritis at an earlier stage than is currently possible.

Item Type:Conference contribution (Poster)
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:24439
Deposited By: Dr David Gibson
Deposited On:07 Jan 2013 10:01
Last Modified:09 Dec 2015 11:10

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