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Difference In-Gel Electrophoresis: A High-Resolution Protein Biomarker Research Tool

Gibson, David (2008) Difference In-Gel Electrophoresis: A High-Resolution Protein Biomarker Research Tool. In: Biomarker Methods in Drug Discovery and Development. Humana Press, USA, pp. 189-209. ISBN 978-1-934115-23-7 [Book section]

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Difference in-gel electrophoresis (DIGE) is a recent adaptation of conventional two-dimensional gel electrophoresis (2-DE) that incorporates novel fluorescent labels, has multiplex attributes, and boasts software-assisted image analysis. Combined, these characteristics offer significant benefits in accuracy and reproducibility to quantify differential protein expression levels between biological samples. The DIGE technique and materials required to perform it are described in detail within. The principles behind consistent gel image acquisition and reliable image analysis are also considered. Within the context of biomarker and drug target discovery, this method simplifies analysis, increases sample throughput, and represents a reliable 2-DE platform.

Item Type:Book section
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:24440
Deposited By: Dr David Gibson
Deposited On:07 Jan 2013 10:01
Last Modified:09 Dec 2015 11:10

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