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Proteomic analysis of synovial fluid and plasma from horses fed a high or low starch diet.

Gibson, David (2010) Proteomic analysis of synovial fluid and plasma from horses fed a high or low starch diet. In: American Society of Animal Science Joint Annual Meeting, Denver, Colorado. American Society of Animal Science. Vol 88 (s2) 4 pp. [Conference contribution]

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URL: http://www.jtmtg.org/2010/abstracts/0761.pdf


A tentative link has been suggested between osteochondrosis (OC) andfeeding high starch diets in growing horses. The pathology of OC ismultifactorial and it is therefore appropriate to explore using a techniquethat allows for characterization and quantification of the plasma (PL)and synovial fluid (SF) proteomes. The objective of this study was todetermine if the concentration of dietary starch influences the proteomesof SF and PL and to isolate, identify and characterize proteins that aredifferentially present in relation to diet or sample type. Six yearlings,maintained on dry lot with ad libitum access to <5% starch hay, wereused in this crossover study design conducted over 12 weeks with four21-d periods. During periods 2 and 4, yearlings were split into 2 treatmentgroups, with one group receiving high starch feed (~40% starch ona DM basis)(HSF) and the other group receiving low starch feed (<10%starch on a DM basis)(LSF). All yearlings received LSF in periods 1and 3. SF and PL samples were taken every 21 d and analyzed using2D gel electrophoresis. Progenesis SameSpots software was used toanalyze spot intensities using ANOVA and differences were consideredsignificant at P < 0.05. Select spots were chosen for mass spectrometry.Changes in protein expression ranged from a 1.2 fold–2.5 fold change.Seven proteins were higher as a result of dietary treatment. Four of the7 proteins, identified as isoforms of immunoglobulin gamma, werehigher in SF from animals fed HSF. Two proteins, identified as isoformsof albumin, were higher in HSF samples. Fibrinogen, haptoglobin andclusterin were higher in PL. Alpha 1 antitrypsin was higher in SF. Thegrouping of isoforms and proteins with similar characteristics on thegel was useful in achieving the goals of this study. Proteins identified inthis study initially do not have a clear mechanistic link with OC. Thisstudy demonstrates a novel approach to investigate protein expressionchanges in PL and SF that may affect joint health. The fluid specificproteomic profiles and individual proteins identified may be useful infuture examination of equine biological fluids.

Item Type:Conference contribution (Speech)
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:24625
Deposited By: Dr David Gibson
Deposited On:21 Jan 2013 12:56
Last Modified:09 Dec 2015 11:10

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