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The use of oxygen uptake rate measurements to control the supply of toxic substrate: toluene hydroxylation by Pseudomonas putida UV4.

Hack, Catherine (2001) The use of oxygen uptake rate measurements to control the supply of toxic substrate: toluene hydroxylation by Pseudomonas putida UV4. Enzyme and microbial technology, 28 (2-3). pp. 183-188. [Journal article]

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Abstract

During toluene hydroxylation, catalyzed by Pseudomonas putida UV4 one molecule of oxygen is added to the aromatic ring to produce the dihydroxylated (non-aromatic) ring structure, toluene cis-glycol. Toluene, which is toxic to the cells at aqueous phase concentration above ( approximately 2.4 mmol), is fed to the reactor. A feed-back control system based on oxygen uptake rate measurements was used to control the feed rate, and thus maintain the aqueous phase toluene concentration in the desired range for zero order kinetics.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
ID Code:25739
Deposited By: Dr Catherine Hack
Deposited On:23 Apr 2013 09:52
Last Modified:23 Apr 2013 09:52

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