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Effects of α-Lipoic Acid on Peripheral Mononuclear Cell and Mitochondrial DNA Damage after Isolated Muscle Contractions

Fogarty, MC, Devito, G, Hughes, CM, Burke, G, Brown, JC, McEneny, J, Brown, D, McClean, Conor and Davison, Gareth (2013) Effects of α-Lipoic Acid on Peripheral Mononuclear Cell and Mitochondrial DNA Damage after Isolated Muscle Contractions. Medicine & Science in Sports & Exercise, 45 (8). pp. 1469-1477. [Journal article]

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URL: http://www.acsm.org/access-public-information/acsm-journals/medicine-science-in-sports-exercise

DOI: 10.1249/MSS.0b013e31828bf31e

Abstract

Introduction: Although pharmacological antioxidants have previously been investigated for a prophylactic effect against exercise oxidative stress, is not known if α-lipoic acid supplementation can protect against DNA damage following high intensity isolated quadriceps exercise. This randomised controlled investigation was designed to test the hypothesis that fourteen days of α-lipoic acid supplementation can attenuate exercise-induced oxidative stress. Methods: Twelve (n=12) apparently healthy male participants (age 28 ± 10 years, stature 177 ± 12 cm and body mass 81 ± 15 kg) were randomly assigned to receive either a daily supplement of 1000mg of α-lipoic acid (2 × 500mg tablets) for fourteen days (n = 6) or receive no supplement (n = 6) in a double blinded experimental approach. Blood and muscle biopsy tissue samples were taken at rest and following the completion of 100 isolated and continuous maximal knee extension (minimum force = 200 N, speed of contraction = 60°/sec). Results: Exercise increased mitochondrial 8-OHdG concentration in both groups (P < 0.05 vs. rest) with a concomitant decrease in total antioxidant capacity (P < 0.05 vs. rest). There was a marked increase in blood total antioxidant capacity following oral α-lipoic acid supplementation (P < 0.05 vs. non-supplemented), while DNA damage (Comet assay and 8-OHdG) lipid peroxidation and hydrogen peroxide increased following exercise in the non-supplemented group only (P < 0.05 vs. supplemented). Exercise increased protein oxidation in both groups (P < 0.05 vs. rest). Conclusion: These findings suggest that short-term α-lipoic acid supplementation can selectively protect DNA (but not in muscle mitochondria) and lipids against exercise-induced oxidative stress.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Sport
Faculty of Life and Health Sciences
Research Institutes and Groups:Sport and Exercise Sciences Research Institute
Sport and Exercise Sciences Research Institute > Centre for Physical Activity and Health
ID Code:25942
Deposited By: Miss Aideen Reilly
Deposited On:02 May 2013 10:47
Last Modified:25 Sep 2013 11:44

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