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Through the eye of an electrospray needle: mass spectrometric identification of themajor peptides and proteins in the milk of the one-humped camel (Camelus dromedarius)

Gibson, David (2013) Through the eye of an electrospray needle: mass spectrometric identification of themajor peptides and proteins in the milk of the one-humped camel (Camelus dromedarius). Journal of Mass Spectrometry, 48 (7). pp. 779-794. [Journal article]

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URL: http://onlinelibrary.wiley.com/doi/10.1002/jms.3213/abstract;jsessionid=3E3F524FA6C858EDD64775837AA01F20.d03t04

Abstract

The milk of the one-humped camel (Camelus dromedarius) reportedly offers medicinal benefits, perhaps because of its unique bioactive components. Milk proteins were determined by (1) two-dimensional gel electrophoresis and peptide mass mapping and (2) liquid chromatography-tandem mass spectrometry (LC-MS/MS) following one-dimensional polyacrylamide gel electrophoresis. Over 200 proteins were identified: some known camel proteins including heavy-chain immunoglobulins and others exhibiting regions of exact homology with proteins from other species. Indigenous peptides were also identified following isolation and concentration by two strategies: (1) gel-eluted liquid fraction entrapment electrophoresis and (2) small-scale electrophoretic separation. Extracts were analyzed by LC-MS/MS and peptides identified by matching strategies, by de novo sequencing and by applying a sequence tag tool requiring similarity to the proposed sequence, but not an exact match. A plethora of protein cleavage products including some novel peptides were characterized. These studies demonstrate that camel milk is a rich source of peptides, some of which may serve as nutraceuticals.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:26473
Deposited By: Dr David Gibson
Deposited On:23 Jul 2013 09:40
Last Modified:15 Oct 2013 14:49

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