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Enzyme induction and cytotoxicity of phenethyl isothiocyanate and its glutathione conjugate towards breast cancer cells

Lam, Yui Sze and Owusu-Apenten, Richard (2013) Enzyme induction and cytotoxicity of phenethyl isothiocyanate and its glutathione conjugate towards breast cancer cells. Pure and Applied Chemical Sciences, 1 (2). pp. 63-73. [Journal article]

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URL: http://www.m-hikari.com/pacs/pacs2013/1-4-2013/3514.html

DOI: http://dx.doi.org/10.12988/pacs.2013.3514

Abstract

Chemoprevention using isothiocyanates is partly the result of the induction ofphase II enzymes for carcinogen detoxification from healthy cells. However,phase II enzyme activity can impair cancer therapeutic agents. The objective ofthis study was to assess phase II enzyme induction and cytotoxicity of phenethylisothiocyanate (PEITC) and glutathione conjugate with PEITC (GsPEITC) usingMCF-7 and MDA-MB-231 breast cancer cells. Changes of phase II enzymes,glutathione-S-transferase (GST) and NAD(P)H quinone reductase (QR), weremeasured by colorimetric procedures. Cell viability was determined using theMTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium- bromide) assay.From current results, 24hr exposure to <5 μM of PEITC or GsPEITC was notcytotoxic to breast cancer cells. No significant induction of phase II enzymeoccurred with a low dose range. By contrast, MCF-7 viability decreased by 50%following 24 hr treatment with 42 ±0.5 μM PEITC or 19. ±0.1 μM GsPEITC. Theconcentrations for 50% loss of viability (IC50) were 103±0.5 μM PEITC and32±0.5 μM GsPEITC in tests using MDA-MB-231 cells. Thus, MCF-7 cells weresignificantly more sensitive to PEITC or GsPEITC compared to MDA-MB-231cells. Cytotoxic doses (>50 μM) of PEITC or GsPEITC increased phase IIenzyme activity by a maximum of 500-700-percent. To conclude, cytotoxic dosesof PEITC or GsPEITC possess phase II enzyme inducing activity in MCF-7 andMDA-MB-231 breast cancer cells.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
ID Code:26709
Deposited By: Dr Richard Owusu-Apenten
Deposited On:03 Sep 2013 13:28
Last Modified:17 Oct 2017 16:10

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