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Mass Spectrometry for Peptide and Protein Analysis

Duncan, Mark and Gibson, David (2012) Mass Spectrometry for Peptide and Protein Analysis. In: Encyclopedia of Analytical Chemistry. John Wiley & Sons, Ltd., Encyclopedia of Analytical Chemistry, pp. 1-22. ISBN ISBN: 9780470027318 [Book section]

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URL: http://onlinelibrary.wiley.com/doi/10.1002/9780470027318.a9199/abstract

DOI: 10.1002/9780470027318.a9199

Abstract

Mass spectrometry is playing an increasingly important role in the characterization and quantification of peptides and proteins. In fact, more conventional approaches to characterization, including techniques such as Edman sequencing and amino acid (AA) analysis, have been largely displaced. Mass analysis — following either matrix-assisted laser desorption ionization (MALDI) or electrospray ionization (ESI) — offers rapid molecular mass determinations with high mass accuracy on subpicomole amounts of material and information can be obtained on the constituents of complex mixtures.The application of tandem mass spectrometry (MS/MS) allows fragmentation of peptide ions, and of proteins with some enhancements too. The MS/MS spectra of peptides and proteins can interpreted de novo or matched against database entries to provide partial amino acid sequence information and peptide/protein identification. Both peptide-centric (bottom-up) and protein-centric (top-down) approaches to protein characterization have been adopted and are now routinely applied.Mass spectrometry has also been pivotal to the development and wide application of proteomics: i.e. the large-scale identification of the proteins present in a complex biological sample. Proteomics is now routinely applied to characterize and quantify the constituents of complex biological tissues and fluids.This entry describes the available approaches to peptide and protein characterization based on mass spectrometry, the constantly evolving analytical methods for proteomics, and available strategies for protein quantification.

Item Type:Book section
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:26743
Deposited By: Dr David Gibson
Deposited On:17 Sep 2013 11:37
Last Modified:15 Oct 2013 14:50

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