Singh - Nee Nigam, Poonam and Marchant, R (1993) PRODUCTION OF THE ENZYME DIHYDROFOLATE-REDUCTASE BY METHOTREXATE-RESISTANT BACTERIA ISOLATED FROM SOIL. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 56 (1). pp. 35-40. [Journal article]
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Six bacterial cultures isolated from soil were capable of growing in the presence of methotrexate (MTX). Two strains, PFR-1 and 3, developed resistance to 500 mug cm-3 MTX in the medium and produced elevated levels of the enzyme dihydrofolate reductase (EC 1. 5.1. 3): 2580 and 2702 U dm-3 compared to the sensitive parent strains (28 and 35 U dm-3). Isolate PFR-3 showed maximum enzyme production (4950 U dm-3, specific activity 12.56 U mg-1 in flasks and 5737 U dm-3, specific activity 14.80 U mg-1 in 5-dm3 fermenter) during exponential phase of growth (6 h) at 37-degrees-C and pH 7.0.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences > School of Biomedical Sciences|
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
|Deposited By:||Professor Roger Marchant|
|Deposited On:||15 Dec 2009 09:31|
|Last Modified:||04 Aug 2011 08:49|
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