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Evaluation of glycated insulin in diabetic animals using immunocytochemistry and radioimmunoassay

McKillop, Aine, Mooney, MH, Harriott, P, Flatt, Peter and O'Harte, Finbarr (2001) Evaluation of glycated insulin in diabetic animals using immunocytochemistry and radioimmunoassay. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 286 (3). pp. 524-528. [Journal article]

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Glycated insulin was evaluated in plasma and biological tissues of diabetic animal models by immuno. cytochemistry (ICC) and a novel radioimmunoassay. Glycated insulin circulated at 0.10 +/-0.04 ng/ml and 2.20 +/-0.14 ng/ml in lean and diabetic obese (ob/ob) mice, corresponding to 12.5 and 9.8% total plasma insulin, respectively. The concentration of glycated insulin was elevated 22-fold in obese mice compared to controls (P<0.001). In the pancreas, glycated insulin was 48 +/-10 and 83 +/- 4 ng/g wt (P<0.05) in lean and obese mice, respectively, representing approximately 2% total insulin in the diabetic pancreas (4.60 plus/minus 0.17 mug/g wt). ICC revealed fluorescent positively stained cells in pancreatic islets from hydrocortisone (HC)treated diabetic rats. Fasting of HC-treated rats, resulted in 3-fold and 15-fold reductions in plasma glycated insulin (P<0.01) and insulin (P<0.001), respectively. Following a 30 min feeding period in these insulin resistant rats, plasma glucose, insulin, and glycated insulin increased (P<0.001) rapidly with 1.4-, 1.6-, and 2.9-fold elevations, respectively. Injection of HC-treated rats with insulin (50 U/kg) resulted in a rapid 33% decrease of plasma glucose (P<0.001) and a marked 4-fold increase in plasma insulin (P<0.01), whereas glycated insulin concentrations remained unchanged. Since glycation of insulin impairs biological activity, physiologically regulated secretion of glycated insulin into the circulation in diabetic animal models suggests a role in the pathogenesis of diabetes. (C) 2001 Academic Press.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
ID Code:3079
Deposited By: Professor Peter Flatt
Deposited On:14 Dec 2009 16:39
Last Modified:09 May 2016 10:48

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