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Anti-viral antibody profiling by high density protein arrays.

Bian, X, Wiktor, P, Kahn, P, Khela, A, Karthikeyan, K, Barker, K, Yu, X, Magee, M, Wasserfall, CH, Gibson, David, Rooney, ME, Qiu, J and LaBaer, J (2015) Anti-viral antibody profiling by high density protein arrays. Proteomics, Tbc . [Journal article]

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DOI: doi: 10.1002/pmic.201400612


Viral infections elicit anti-viral antibodies and have been associated with various chronic diseases. Detection of these antibodies can facilitate diagnosis, treatment of infection and understanding of the mechanisms of virus associated diseases. In this work, we assayed anti-viral antibodies using a novel high density-nucleic acid programmable protein array (HD-NAPPA) platform. Individual viral proteins were expressed in situ directly from plasmids encoding proteins in an array of microscopic reaction chambers. Quality of protein display and serum response was assured by comparing intra- and inter- array correlation within or between printing batches with average correlation coefficients of 0.91 and 0.96, respectively. HD-NAPPA showed higher signal to background (S/B) ratio compared with standard NAPPA on planar glass slides and ELISA. Antibody responses to 761 antigens from 25 different viruses were profiled among patients with juvenile idiopathic arthritis (JIA) and type 1 diabetes (T1D). Common as well as unique antibody reactivity patterns were detected between patients and healthy controls. We believe HD-viral-NAPPA will enable the study of host-pathogen interactions at unprecedented dimensions and elucidate the role of pathogen infections in disease development. This article is protected by copyright. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:31190
Deposited By: Dr David Gibson
Deposited On:26 Mar 2015 11:59
Last Modified:26 Mar 2015 11:59

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