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Isolation and characterisation of insulin-releasing compounds from Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis venom

Moore, Sara W.M., Bhat, Vikas K., Flatt, Peter, Gault, Victor and McClean, Stephen (2015) Isolation and characterisation of insulin-releasing compounds from Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis venom. Toxicon, 101 . 48 - 54. [Journal article]

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URL: http://www.sciencedirect.com/science/article/pii/S0041010115001257

DOI: http://dx.doi.org/10.1016/j.toxicon.2015.05.002

Abstract

Crude venom from three venomous snakes, Crotalus adamanteus, Crotalus vegrandis and Bitis nasicornis was fractionated by gel filtration chromatography, and selected fractions screened for in-vitro insulinotropic activity using clonal pancreatic BRIN-BD11 cells. Nineteen fractions stimulated insulin secretion and the structural identity of bioactive compounds responsible was probed using MALDI-ToF {MS} and N-terminal Edman degradation sequencing. Partial N-terminal sequences were determined and their homology to existing sequences identified using {BLAST} searching. The main insulinotropic peptide families identified were made up of snake venom serine proteinases, phospholipases {A2} (PLA2) and disintegrins. Snake venom constituents may have therapeutic potential for diabetes, with each of the three viper venoms tested providing insulinotropic compounds from a range of different toxin families.

Item Type:Journal article
Keywords:Diabetes
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
Biomedical Sciences Research Institute > Diabetes
ID Code:31632
Deposited By: Dr Stephen McClean
Deposited On:19 May 2015 08:30
Last Modified:28 Jul 2015 15:20

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