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Kinetics and thermodynamics of human serum albumin adsorption on silicon doped diamond like carbon

Ahmed, MH, Byrne, JA and McLaughlin, JAD (2015) Kinetics and thermodynamics of human serum albumin adsorption on silicon doped diamond like carbon. Materials Chemistry and Physics, 154 . pp. 84-93. [Journal article]

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URL: http://dx.doi.org/10.1016/j.matchemphys.2015.01.048

DOI: 10.1016/j.matchemphys.2015.01.048


To gain a better understanding of protein adsorption onto biomaterial surfaces is required for the control of biocompatibility and bioactivity. Various samples of diamond like carbon (DLC) and silicon-doped DLC were synthesised using plasma enhanced chemical vapour deposition (PECVD). The effects of surface morphology on the interaction of human serum albumin (HSA) with doped and undoped DLC films was investigated using spectroscopic ellipsometry (SE) and other surface analysis techniques. The results highlighted an increase in both contact angle and hydrophobicity with increasing silicon dopant levels. A reduction on the contact angle values.After adsorption of HSA, the films showed a reduction in the contact angle with a significant change in the cosΔ and this gap increased with increasing surface coverage of HSA. The adsorption kinetics of HSA were also investigated and revealed that the maximum adsorption occurred at pH 5.0 and the process involved chemisorption. The experimental isotherm data were analysed using the Langmuir and Freundlich‎ models. The amount of HSA adsorbed increased with contact time and reached saturation ‎after 30 min. The adsorption ‎process was found to be pseudo first order with respect to the bulk concentration and was dependent on both the concentration of protein and surface characteristics of the samples. The amount of adsorbed HSA was higher with higher levels of silicon doping of the DLC. Therefore, doping DLC may provide an approach to controlling the protein adsorption.

Item Type:Journal article
Keywords:adsorption; biomaterials; chemical vapour deposition; thermodynamic
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:31997
Deposited By: Mrs Ann Blair
Deposited On:01 Jul 2015 10:16
Last Modified:08 Sep 2017 14:18

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