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Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)

Metcalfe, Richard, Koumanov, Francoise, Ruffino, Jose, Stokes, Keith, Holman, Geoff, Thomspon, Dylan and Vollaard, Niels (2015) Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT). European Journal of Applied Physiology, 2015 . [Journal article]

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DOI: 10.1007/s00421-015-3217-6

Abstract

Purpose: We have previously shown that six weeks of reduced-exertion high-intensity interval training (REHIT) improves V̇O2max in sedentary men and women, and insulin sensitivity in men. Here we present two studies examining the acute physiological and molecular responses to REHIT.Methods: In Study 1, five men and six women (age: 26±7 y, BMI: 23±3 kg∙m-2, V̇O2max: 51±11 ml∙kg-1∙min-1) performed a single 10-min REHIT cycling session (60 W and two 20-s ‘all-out’ sprints), with vastus lateralis biopsies taken before and 0, 30 and 180 min post-exercise for analysis of glycogen content, phosphorylation of AMPK, p38 MAPK and ACC, and gene expression of PGC1α and GLUT4. In Study 2, eight men (21±2 y; 25±4 kg·m-2; 39±10 ml·kg-1·min-1) performed three trials (REHIT, 30 min cycling at 50% of V̇O2max, and a resting control condition) in a randomised cross-over design. Expired air, venous blood samples, and subjective measures of appetite and fatigue were collected before and 0, 15, 30 and 90 min post-exercise.Results: Acutely, REHIT was associated with a decrease in muscle glycogen, increased ACC phosphorylation, and activation of PGC1α. When compared to aerobic exercise, changes in V̇O2, RER, plasma volume, and plasma lactate and ghrelin were significantly more pronounced with REHIT, whereas plasma glucose, NEFAs, PYY, and measures of appetite were unaffected.Conclusions: Collectively these data demonstrate that REHIT is associated with a pronounced disturbance of physiological homeostasis and associated activation of signalling pathways, which together may help explain previously observed adaptations once considered exclusive to aerobic exercise.

Item Type:Journal article
Keywords:HIT, Glycogen, Signalling pathways, AMPK, Exercise metabolism, Energy balance
Faculties and Schools:Faculty of Life and Health Sciences > School of Sport
Faculty of Life and Health Sciences
Research Institutes and Groups:Sport and Exercise Sciences Research Institute
Sport and Exercise Sciences Research Institute > Centre for Physical Activity and Health
ID Code:32088
Deposited By: Mr Richard Metcalfe
Deposited On:15 Jul 2015 10:18
Last Modified:17 Oct 2017 16:19

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