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Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health.

Brown, Emma, Nitecki, Sonja, Pereira-Caro, Gemma, Mc Dougall, Gordon, Stewart, Derek, Crozier, Alan, Rowland, ian and Gill, Chris IR (2014) Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health. Biofactors., 40 (6). pp. 611-623. [Journal article]

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DOI: doi: 10.1002/biof.1173

Abstract

The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated in vitro digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC-PDA-MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the in vivo and in vitro digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (P < 0.05) anti-genotoxic (Comet assay), anti-mutagenic (Mutation Frequency assay), and anti-invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically-relevant doses (0-50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti-genotoxic activity (P < 0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity in vitro. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals.

Item Type:Journal article
Keywords:Polyphenols, in vitro digestion, ileal fluid, diet, gut health, lingonberries, DNA damage, in vivo digestion
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
ID Code:32186
Deposited By: Dr Chris Gill
Deposited On:03 Sep 2015 09:45
Last Modified:03 Sep 2015 09:45

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