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Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom

Moore, Sara W.M., Bhat, Vikas K., Flatt, Peter, Gault, Victor and McClean, Stephen (2016) Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom. International Journal of Peptide Research and Therapeutics, 22 . pp. 211-218. [Journal article]

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URL: http://dx.doi.org/10.1007/s10989-015-9499-z

DOI: 10.1007/s10989-015-9499-z

Abstract

Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P < 0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.

Item Type:Journal article
Keywords:Pseudechis australis; Pseudechis butleri; Elapid; Venom; Insulin secretion; Diabetes
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Diabetes
ID Code:32770
Deposited By: Dr Stephen McClean
Deposited On:08 Dec 2015 11:25
Last Modified:21 Jun 2016 08:36

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