Moore, Sara W.M., Bhat, Vikas K., Flatt, Peter, Gault, Victor and McClean, Stephen (2016) Isolation and Characterisation of Insulin-Releasing Compounds from Pseudechis australis and Pseudechis butleri Venom. International Journal of Peptide Research and Therapeutics, 22 . pp. 211-218. [Journal article]
- Accepted Version
Restricted to Repository staff only until 1 July 2017.
Crude venom from two elapid snakes Pseudechis australis and Pseudechis butleri was fractionated by gel filtration chromatography and selected fractions screened for in vitro insulin-releasing activity using clonal pancreatic BRIN-BD11 cells. Following acute 20-min incubation at 5.6 mM glucose, 9 fractions exhibited significant (P < 0.001) insulin-releasing activity. Structural characterisation of active fractions was achieved primarily using MALDI–TOF MS and N-terminal Edman degradation sequencing. The partial N-terminal sequences are reported for a total of 7 venom components. Their homology to existing sequences as determined using BLAST searching uncovered the main insulin-releasing families as being phospholipases A2 and short α-neurotoxins. A number of sequences are reported for the first time from P. butleri venom which is much less studied than the related P. australis.
|Item Type:||Journal article|
|Keywords:||Pseudechis australis; Pseudechis butleri; Elapid; Venom; Insulin secretion; Diabetes|
|Faculties and Schools:||Faculty of Life and Health Sciences > School of Biomedical Sciences|
Faculty of Life and Health Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Biomedical Sciences Research Institute > Diabetes
|Deposited By:||Dr Stephen McClean|
|Deposited On:||08 Dec 2015 11:25|
|Last Modified:||21 Jun 2016 08:36|
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