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Protein adsorption on nano-patterned hydrogenated amorphous carbon model surfaces

Mughal, MZ, Lemoine, P, Lubarsky, G and Maguire, PD (2016) Protein adsorption on nano-patterned hydrogenated amorphous carbon model surfaces. Materials and Design, 97 (5). pp. 239-248. [Journal article]

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URL: http://www.sciencedirect.com/science/article/pii/S0264127516301897

DOI: 10.1016/j.matdes.2016.02.043

Abstract

Predicting how proteins fold and adsorb onto surfaces is a complex problem of strong relevance to the health and environmental sectors. In this work, two nano-patterning techniques, namely focused ion beam (FIB) milling and atomic force microscopy (AFM) nanoindentation were used to develop hydrogenated amorphous carbon (a-C:H) model surfaces with similar nano-topography but different local composition. On the un-patterned surfaces, bovine plasma fibrinogen (BPF) resulted in a thicker and rougher adsorbed film than bovine serum albumin (BSA), although FTIR analysis indicated that, the secondary structure of the proteins changed similarly, with an increase of the -sheet component (+27% and +34% for BSA and BPF, respectively). AFM analysis on the FIB-patterned surfaces indicates that patterning can modify specific protein adsorption behaviors. Moreover, the patterns were compared by imaging the AFM tip/surface adhesive force for BSA adsorbed on either AFM tips or patterned surfaces. The results shows an electrostatic interaction between the implanted Ga+ and BSA surface, modifying the adsorption behavior and the adhesive force. Modelling this interaction gave an estimate of the surface charge per protein, a significantly lower value than in dilute solution (-1.8e instead of -18e). This finding is indicative of protein misfolding, as detected in the FTIR analysis.

Item Type:Journal article
Keywords:Focused ion beam (FIB) Atomic force microscopy (AFM) nanoindentation Nano-patterning Hydrogenated amorphous carbon Protein adsorption
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:34156
Deposited By: Dr Patrick Lemoine
Deposited On:11 Apr 2016 08:09
Last Modified:09 Feb 2017 09:51

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