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Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells

Gimenez-Abian, JF, Clarke, DJ, de la Torre, C, Gimenez-Martin, G, Mullinger, AM, Downes, Stephen and Johnson, RT (1999) Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells. EUROPEAN JOURNAL OF CELL BIOLOGY, 78 (8). pp. 601-603. [Journal article]

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Condensed sister chromatids possess a protein scaffold or axial core to which loops of chromatin are attached. The sister cores are believed to be dynamic frameworks that function in the organization and condensation of chromatids. Chromosome structural proteins are implicated in the establishment of sister chromatid cohesion and in the maintenance of epigenetic phenomena. Both processes of templating are tightly linked to DNA replication itself. It is a question whether the structural basis of sister chromatid cores is templated during S phase. As cells proceed through the cell cycle, chromatid cores undergo changes in their protein composition. Cytologically, cores are first visualized at the start of prometaphase, Still, core assembly can be induced in G(1) and G(2) when interphase cells are fused with mitotic cells. In this study, we asked if chromatid cores are similarly able to assemble in S-phase cells. We find that the ability to assemble cores is transiently lost during local replication, then regained in chromosome regions shortly after they have been replicated. We propose that core templating occurs coincident with DNA replication and that the competence for the assembly of the sister chromatid cores is acquired shortly after passage of replication forks.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Genomic Medicine
ID Code:3507
Deposited By: Professor Stephen Downes
Deposited On:15 Dec 2009 14:34
Last Modified:08 May 2017 16:10

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