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Proposal to reclassify Propionibacterium acnes type I as Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes type II as Propionibacterium acnes subsp. defendens subsp. nov.

McDowell, Andrew, Barnard, Emma, Liu, Jared, Li, Huiying and Patrick, Sheila (2016) Proposal to reclassify Propionibacterium acnes type I as Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes type II as Propionibacterium acnes subsp. defendens subsp. nov. International Journal of Systematic and Evolutionary Microbiology, 66 . pp. 5358-5365. [Journal article]

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DOI: 10.1099/ijsem.0.001521

Abstract

Recently, strains of Propionibacterium acnes from the type III genetic division have been proposed as Propionibacterium acnes subsp. elongatum subsp. nov., with strains from the type I and II divisions collectively classified as Propionibacterium acnes subsp. acnes subsp. nov. Under such a taxonomic re-appraisal, we believe that types I and II should also have their own separate rank of subspecies. In support of this, we describe a polyphasic taxonomic study based on the analysis of publically available multilocus and whole genome sequence datasets, alongside a systematic review of previously published phylogenetic, genomic, phenotypic and clinical data. Strains of types I and II form highly distinct clades based on multilocus sequence analysis (MLSA) and whole genome phylogenetic reconstructions. In silico or digital DNA-DNA similarity values also fall within the 70-80% boundary recommended for bacterial subspecies. Furthermore, we see important differences in genome content, including the presence of an active CRISPR/Cas system in type II strains, but not type I, and evidence for increasing linkage equilibrium within the separate divisions. Key biochemical differences include positive tests for beta-haemolytic, neuraminidase and sorbitol fermentation activities with type I strains, but not type II. We now propose type I strains as Propionibacterium acnes subsp. acnes subsp. nov., and type II as Propionibacterium acnes subsp. defendens subsp. nov. The type strain of Propionibacterium acnes subsp. acnes subsp. nov. is NCTC 737T (=ATCC 6919T=JCM 6425T=DSM 1897T=CCUG 1794T), while the type strain of Propionibacterium acnes subsp. defendens subsp. nov. is ATCC 11828 (=JCM 6473=CCUG 6369).

Item Type:Journal article
Keywords:Propionibacterium acnes subsp. defendens subsp. nov., type I, type II, Multilocus Sequence Analysis, Genomics, Phenotype
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:36109
Deposited By: Dr Andrew McDowell
Deposited On:18 Oct 2016 08:34
Last Modified:01 Dec 2017 23:23

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