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Rapid Quantification and Validation of Lipid Concentrations within Liposomes

Roces, Carla, Kastner, Elizabeth, Stone, Peter, Lowry, Deborah and Perrie, Yvonne (2016) Rapid Quantification and Validation of Lipid Concentrations within Liposomes. Pharmaceutics, 8 (29). pp. 1-11. [Journal article]

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URL: http://www.mdpi.com/1999-4923/8/3/29

DOI: 10.3390/pharmaceutics8030029

Abstract

Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance, since this concentration impacts both efficacy and stability. In this paper, we outline a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method that allows for the rapid and simultaneous quantification of lipid concentrations within liposomal systems prepared by three liposomal manufacturing techniques (lipid film hydration, high shear mixing, and microfluidics). The ELSD system was used to quantify four lipids: 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), cholesterol, dimethyldioctadecylammonium (DDA) bromide, and D-(+)-trehalose 6,6′-dibehenate (TDB). The developed method offers rapidity, high sensitivity, direct linearity, and a good consistency on the responses (R2 > 0.993 for the four lipids tested). The corresponding limit of detection (LOD) and limit of quantification (LOQ) were 0.11 and 0.36 mg/mL (DMPC), 0.02 and 0.80 mg/mL (cholesterol), 0.06 and 0.20 mg/mL (DDA), and 0.05 and 0.16 mg/mL (TDB), respectively. HPLC-ELSD was shown to be a rapid and effective method for the quantification of lipids within liposome formulations without the need for lipid extraction processes.

Item Type:Journal article
Keywords:lipids, liposomes, cholesterol, quantification, HPLC, ELSD
Faculties and Schools:Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Faculty of Life and Health Sciences
ID Code:36304
Deposited By: Dr Deborah Lowry
Deposited On:21 Nov 2016 14:09
Last Modified:17 Oct 2017 16:26

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