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Sonodynamic inactivation of Gram-positive and Gram-negativebacteria using a Rose Bengal–antimicrobial peptide conjugate

Callan, John F, Costley, David, Nesbitt, Heather, Ternan, Nigel, Dooley, James, Huang, Ying-Ying, Hamblin, Michale R and McHale, Anthony P (2017) Sonodynamic inactivation of Gram-positive and Gram-negativebacteria using a Rose Bengal–antimicrobial peptide conjugate. International Journal of Antimicrobial Agents, 49 (1). pp. 31-36. [Journal article]

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URL: http://dx.doi.org/10.1016/j.ijantimicag.2016.09.034

DOI: 10.1016/j.ijantimicag.2016.09.034


Combating antimicrobial resistance is one of the most serious public health challenges facing society today.The development of new antibiotics or alternative techniques that can help combat antimicrobial resistance is being prioritised by many governments and stakeholders across the globe. Antimicrobial photodynamic therapy is one such technique that has received considerable attention but is limited by the inability of light to penetrate through human tissue, reducing its effectiveness when used to treat deep-seated infections. The related technique sonodynamic therapy (SDT) has the potential to overcome this limitation given the ability of low-intensity ultrasound to penetrate human tissue. In this study,a Rose Bengal–antimicrobial peptide conjugate was prepared for use in antimicrobial SDT (ASDT). When Staphylococcus aureus and Pseudomonas aeruginosa planktonic cultures were treated with the conjugate and subsequently exposed to ultrasound, 5 log and 7 log reductions, respectively, in bacterial numbers were observed. The conjugate also displayed improved uptake by bacterial cells compared with a mammalian cell line (P ≤ 0.01), whilst pre-treatment of a P. aeruginosa biofilm with ultrasound resulted in a 2.6-fold improvement in sensitiser diffusion (P ≤ 0.01). A preliminary in vivo experiment involving ASDT treatment of P. aeruginosa-infected wounds in mice demonstrated that ultrasound irradiation of conjugate treated wounds affects a substantial reduction in bacterial burden. Combined, the results obtained from this study highlight ASDT as a targeted broad-spectrum novel modality with potential for the treatment of deep-seated bacterial infections.

Item Type:Journal article
Keywords:Sonodynamic therapy Antimicrobial Sensitiser Peptide
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:36638
Deposited By: Professor John Callan
Deposited On:12 Jan 2017 15:40
Last Modified:26 Jul 2017 14:40

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