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Intracellular mechanisms of solar water disinfection

Castro-Alférez, María, Polo-López, María Inmaculada and Fernandez-Ibanez, Pilar (2016) Intracellular mechanisms of solar water disinfection. Scientific Reports, 6 (38145). pp. 1-10. [Journal article]

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URL: http://dx.doi.org/10.1038/srep38145

DOI: 10.1038/srep38145

Abstract

Solar water disinfection (SODIS) is a zero-cost intervention measure to disinfect drinking water in areas of poor access to improved water sources, used by more than 6 million people in the world. The bactericidal action of solar radiation in water has been widely proven, nevertheless the causes for this remain still unclear. Scientific literature points out that generation of reactive oxygen species (ROS) inside microorganisms promoted by solar light absorption is the main reason. For the first time, this work reports on the experimental measurement of accumulated intracellular ROS in E. coli during solar irradiation. For this experimental achievement, a modified protocol based on the fluorescent probe dichlorodihydrofluorescein diacetate (DCFH-DA), widely used for oxidative stress in eukaryotic cells, has been tested and validated for E. coli. Our results demonstrate that ROS and their accumulated oxidative damages at intracellular level are key in solar water disinfection.

Item Type:Journal article
Keywords:REACTIVE OXYGEN SPECIES; FLOW CITOMETRY, E. COLI
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:36766
Deposited By: Dr Pilar Fernandez-Ibanez
Deposited On:15 Feb 2017 13:30
Last Modified:17 Oct 2017 16:27

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