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Rhamnolipids from Pseudomonas aeruginosa strain W10;as antibiofilm/antibiofouling products for metal protection

Chebbi, Alif, Elshikh, Mohamed, Haque, Farazul, Ahmed, Syed, Dobbin, Sara, Marchant, R, Sayadi, Sami, Chamkha, Mohamed and Banat, Ibrahim M (2017) Rhamnolipids from Pseudomonas aeruginosa strain W10;as antibiofilm/antibiofouling products for metal protection. Journal of Basic Microbiology, 9999 . pp. 1-12. [Journal article]

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DOI: 10.1002/jobm.201600658

Abstract

Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L−1 and a critical micelle concentration (CMC) of 80 mg L−1. P. aeruginosa W10 rhamnolipids were able to disrupt up to 99% of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0.1, 0.5, and 1 mg ml−1 on a stainless steel surface commonly used in industrial process pipelines. CFU measurements and LIVE/DEAD BacLight staining confirmed these observations. Furthermore, a purified di-rhamnolipid fraction was found to be responsible for the microbial inhibition of B. licheniformis strain CAN55. This study providesevidence that rhamnolipids may have valuable applications in preventing biofilms and biofouling in industrial plants and, in a wider context, may also apply to metal medical devices.

Item Type:Journal article
Keywords:biofilm inhibition, biofouling, biosurfactants, rhamnolipids, metal surface
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:37031
Deposited By: Professor Ibrahim Banat
Deposited On:06 Mar 2017 09:10
Last Modified:06 Sep 2017 15:34

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