McGrath, GJ, O'Kane, E, Smyth, Franklin and Tagliaro, F (1996) Investigation of the electrochemical oxidation of clenbuterol at a porous carbon electrode, and its application to the determination of this beta-agonist in bovine hair by liquid chromatography with coulometric detection. ANALYTICA CHIMICA ACTA, 322 (3). pp. 159-166. [Journal article]
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At pH 5, clenbuterol is oxidized at potentials > 650 mV to form a radical cation, two of which combine in a head to head manner to form a dimer. A method for the determination of this notably abused beta(2)-adrenergic drug in bovine hair has been developed, utilizing this oxidative response. After alkaline degradation of the hair, the drug was extracted using a simple liquid-liquid extraction based on hexane, with a recovery of 92 +/- 3.6%. After evaporation to dryness, the residue is dissolved in mobile phase and the drug analysed by reversed phase liquid chromatography using a mobile phase consisting of methanol-0.1 M ammonium acetate (60:40, v/v) with 0.4% sodium dodecyl sulphate adjusted to pH 5 with orthophosphoric acid. Quantitation used a two cell coulometric detector with the first cell being used as a screening electrode and the analytical electrode set at +875 mV.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences|
Faculty of Life and Health Sciences > School of Biomedical Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
|Deposited By:||Prof W.Franklin Smyth|
|Deposited On:||12 Jan 2010 09:42|
|Last Modified:||04 May 2012 08:19|
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