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Targeted drug delivery system to neural cells utilizes the nicotinic acetylcholine receptor

Huey, Rachel, O'Hagan, Barry, McCarron, Paul and Hawthorne, Susan (2017) Targeted drug delivery system to neural cells utilizes the nicotinic acetylcholine receptor. International Journal of Pharmaceutics, 525 (1). pp. 12-20. [Journal article]

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DOI: 10.1016/j.ijpharm.2017.04.023

Abstract

Drug delivery to the brain is still a major challenge in the field of therapeutics, especially for large and hydrophilic compounds. In order to achieve drug delivery of therapeutic concentration in the central nervous system, the problematic blood brain barrier (BBB) must be overcome. This work presents the formulation of a targeted nanoparticle-based drug delivery system using a specific neural cell targeting ligand, rabies virus derived peptide (RDP). Characterization studies revealed that RDP could be conjugated to drug-loaded PLGA nanoparticles of average diameter 257.10 ± 22.39 nm and zeta potential of -5.51 ± 0.73 mV. In vitro studies showed that addition of RDP to nanoparticles enhanced drug accumulation in a neural cell line specifically as opposed to non-neural cell lines. It was revealed that this drug delivery system is reliant upon nicotinic acetylcholine receptor (nAChR) function for RDP-facilitated effects, supporting a cellular uptake mechanism of action. The specific neural cell targeting capabilities of RDP via the nAChR offers a non-toxic, non-invasive and promising approach to the delivery of therapeutics to the brain.

Item Type:Journal article
Keywords:Drug delivery system, rabies virus, targeted nanoparticles, neural, blood brain barrier (BBB), nicotinic acetylcholine receptor
Faculties and Schools:Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Genomic Medicine
Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:37475
Deposited By: Dr Susan Hawthorne
Deposited On:02 May 2017 08:41
Last Modified:06 Sep 2017 14:56

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