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Adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing

Lydon, Helen, Baccile, Niki, Callaghan, Breedge, Marchant, R, Mitchell, CA and Banat, Ibrahim (2017) Adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing. Antimicrobial Agents and Chemotherapy, Epub . AAC.02547-16. [Journal article]

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URL: http://dx.doi.org/10.1128/AAC.02547-16

DOI: 10.1128/AAC.02547-16

Abstract

The sophorolipid class of biosurfactants are finding increasing use in personal care as well as pharmaceutical products and have the potential to disrupt biofilm formation and inhibit growth in a variety of clinically relevant organisms. In order to investigate potential biomedical applications of sophorolipids derived from non-pathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast Starmerella bombicola, which yielded both acidic C 18-1 acetylated and non-acetylated congeners that were essentially free from other contaminants (>95% purity). These acidic sophorolipids have antimicrobial activities against the nosocomial infective agents Enterococcus faecalis and Pseudomonas aeruginosa with significant reduction in c.f.u.'s at concentrations as low as 5 mgmL-1 In addition, the sophorolipid showed similar effects when combined with kanamycin or cefotaxime against the same two bacterial strains. As a potential use of these sophorolipids is as a component of topically applied creams for treatment of wound infections, it is clear that they must have no demonstrable adverse effect on wound healing. To assess this we evaluated mammalian cell toxicity in vitro using viability tests and revealed no adverse effect on either endothelial or keratinocyte derived cell lines with sophorolipid concentrations <0.5 mgmL-1 In addition, in vivo experiments using a mouse skin wounding assay revealed that the time-course in healing wounds was unaffected by application of sophorolipid containing creams and histological examination of regenerated skin tissue confirmed that the healing process was similar to that observed in control animals, with no evidence of inflammation. These results are consistent with the suggestion that acidic sophorolipids can be used as a component of anti-microbial creams to reduce the risk of wound infection during healing.

Item Type:Journal article
Keywords:acidic sophorolipids, microbial pathogens, growth inhibition, endothelium, keratinocytes, in vivo, wound healing
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmacy & Pharmaceutical Sciences
ID Code:37542
Deposited By: Professor Christopher Mitchell
Deposited On:02 May 2017 09:20
Last Modified:17 Oct 2017 16:29

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