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Efficacy of the solar water disinfection method in turbid waters experimentally contaminated withCryptosporidium parvumoocysts under real field conditions

Gómez-Couso, H., Fontán-Saínz, M., Sichel, C., Fernandez Ibanez, P and Ares-Mazás, E. (2009) Efficacy of the solar water disinfection method in turbid waters experimentally contaminated withCryptosporidium parvumoocysts under real field conditions. Tropical Medicine & International Health, 14 (6). pp. 620-627. [Journal article]

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URL: http://dx.doi.org/10.1111/j.1365-3156.2009.02281.x

DOI: 10.1111/j.1365-3156.2009.02281.x

Abstract

bjective To investigate the efficacy of the solar water disinfection (SODIS) method for inactivating Cryptosporidium parvum oocysts in turbid waters using 1.5 l polyethylene terephthalate (PET) bottles under natural sunlight.methods All experiments were performed at the Plataforma Solar de Almerı ́a, located in the Tabernas Desert (Southern Spain) in July and October 2007. Turbid water samples [5, 100 and 300 nephelometric turbidity units (NTU)] were prepared by addition of red soil to distilled water, and then spiked with purified C. parvum oocysts. PET bottles containing the contaminated turbid waters were exposed to full sunlight for 4, 8 and 12 h. The samples were then concentrated by filtration and the oocyst viability was determined by inclusion ⁄ exclusion of the fluorogenic vital dye propidium iodide. results After an exposure time of 12 h (cumulative global dose of 28.28 MJ⁄m2; cumulative UV dose of 1037.06 kJ ⁄ m2) the oocyst viabilities were 11.54%, 25.96%, 41.50% and 52.80% for turbidity levels of 0, 5, 100 and 300 NTU, respectively, being significantly lower than the viability of theinitial isolate (P < 0.01).conclusions SODIS method significantly reduced the potential viability of C. parvum oocysts on increasing the percentage of oocysts that took up the dye PI (indicator of cell wall integrity), although longer exposure periods appear to be required than those established for the bacterial pathogens usually tested in SODIS assays.

Item Type:Journal article
Keywords:SODIS, natural sunlight, PET bottles, turbid waters, Cryptosporidium, viability
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:37911
Deposited By: Dr Pilar Fernandez-Ibanez
Deposited On:30 May 2017 08:21
Last Modified:30 May 2017 08:21

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