Ulster University Logo

A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

Andjelic, Sofija, Lumi, Xhevat, Veréb, Zoltán, Josifovska, Natasha, Facskó, Andrea, Hawlina, Marko and Petrovski, Goran (2014) A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies. Journal of Immunology Research, 2014 . pp. 1-10. [Journal article]

Full text not available from this repository.

URL: http://dx.doi.org/10.1155/2014/232659

DOI: 10.1155/2014/232659

Abstract

A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.

Item Type:Journal article
Keywords:viscoelastic, anterior lens capsule, lens epithelial cells, fibrovascular epiretinal membranes, proliferative diabetic retinopathy, calcium imaging, acetylcholine, mechanostimulation, inflammation, IL-6, IL-8, TNFa
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Optometry and Vision Science
ID Code:38129
Deposited By: Dr Natasha Josifovska
Deposited On:13 Jun 2017 09:26
Last Modified:13 Jun 2017 09:26

Repository Staff Only: item control page