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Application of Liquid Chromatography−Tandem Mass SpectrometryTo Determine Urinary Concentrations of Five Commonly Used Low-Calorie Sweeteners: A Novel Biomarker Approach for Assessing Recent Intakes?

Logue, Caomhan, Dowey, Le Roy, Strain, JJ, Verhagen, Hans, Mcclean, Stephen and Gallagher, Alison (2017) Application of Liquid Chromatography−Tandem Mass SpectrometryTo Determine Urinary Concentrations of Five Commonly Used Low-Calorie Sweeteners: A Novel Biomarker Approach for Assessing Recent Intakes?, Journal of Agricultural and Food Chemistry [Other]

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URL: http://pubs.acs.org/doi/pdf/10.1021/acs.jafc.7b00404

Abstract

Although the use of low-calorie sweeteners (LCSs) is widespread, methods of assessing consumption within free-living populations have inherent limitations. Five commonly consumed LCSs, namely, acesulfame-K, saccharin, sucralose, cyclamate, and steviol glycosides, are excreted via the urine, and therefore a urinary biomarker approach may provide more objective LCS intake data. A LC-ESI-MS/MS method of simultaneously determining acesulfame-K, saccharin, sucralose, cyclamate, and the excretory metabolite of steviol glycosides, steviol glucuronide, in human urine was developed and validated. Linearity was observed over a concentration range of 10−1000 ng/mL with coefficients of determination ranging from 0.9969 to0.9997. Accuracy ranged from 92 to 104%, and intrabatch and interday precisions were within acceptable limits with %CV below 8% for all compounds. A double-blind, randomized crossover dose−response study was conducted to assess the usefulness of urinary LCS excretions (from both fasting spot and a full 24-h urine collection) for investigating recent intakes. Both modes ofsampling were useful for distinguishing between the three short-term intakes of acesulfame-K, saccharin, cyclamates, and steviol glycosides (p < 0.001), whereas for sucralose, urinary concentrations were useful for distinguishing between low (0.1% ADI) and high doses (10% ADI) only (p < 0.001). In summary, this biomarker approach may be useful for assessing intakes of fivecommonly consumed LCSs.

Item Type:Other
Keywords:low-calorie sweeteners, intense sweeteners, biomarkers, urinary biomarkers, exposure, food additives, human urine
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Diabetes
ID Code:38173
Deposited By: Dr Caomhan Logue
Deposited On:29 Jun 2017 09:36
Last Modified:26 Jun 2019 14:13

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