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Methotrexate combination effects with genistein and daidzein on MDA-MB-231 breast cancer cell viability

Maginnes, Ashleigh and Owusu-Apenten, Richard K. (2017) Methotrexate combination effects with genistein and daidzein on MDA-MB-231 breast cancer cell viability. Journal of Applied Life Sciences International, 13 (1). pp. 1-9. [Journal article]

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Aims: To investigate the effect of methotrexate and soy isoflavones genistein and daidzein on MDA-MB-231 breast cancer cell viability. Study Design: In-vitro study using cultured cellsPlace and Duration of Study: Nutrition Innovations Centre for Food & Health (NICHE), School of Biomedical Sciences, Ulster University, data collected September 2014-2015.Methods: Human breast cancer MDA-MB-231 cells were cultured in DMEM (with 10% FBS, 1% Pen strep) and treated with methotrexate, genistein or daidzein for 72 hrs. Combinations treatments used non-fixed ratios of methotrexate (0-100 μM) and genistein or daidzein (30 μM) with cell viability monitored using the MTT assay. Results: The 50% effect dose (EC50) was 44.7±6.4 μM for methotrexate, 55.8±3.9 μM for genistein or 67.4±12.2 μM for daidzein. Combination treatments with genistein or daidzein produced EC50 of 57.6±2.0 or 29.7±2.4 μM for methotrexate, respectively. The combination index (CI) was 1.9 for methotrexate-genistein whilst CI was 1.1 for methotrexate-daidzein near the median dose. Values for CI decreased from 5.0 towards 1.0 as the ratio of methotrexate: isoflavone increased. The results are discussed in terms of prevailing ideas concerning how phytochemicals affects drug adsorption, distribution, metabolism and excretion (ADME) and the expected consequences for cytotoxicity.Conclusions: Treatment of MDA-MB-231 breast cancer cells with methotrexate and genistein or daidzein produces interactions consistent with antagonism (CI =1.1-5.0) but the effects are predicted to diminish with rising methotrexate to isoflavone ratio.

Item Type:Journal article
Keywords:Methotrexate; breast cancer; isoflavones; genistein; daidzein; interactions.
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
ID Code:38392
Deposited By: Dr Richard Owusu-Apenten
Deposited On:10 Aug 2017 10:20
Last Modified:10 Aug 2017 22:23

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