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miR-199a-5p is a marker of blood pressure in premature cardiovascular disease patients homozygous for the MTHFR C677T polymorphism.

Lynch, Seodhna, Ward, Mary, McNulty, Helene, Horigan, G, Strain, JJ, purvis, John, tackett, Mike and McKenna, Declan (2017) miR-199a-5p is a marker of blood pressure in premature cardiovascular disease patients homozygous for the MTHFR C677T polymorphism. Journal of Human Hypertension, 31 . S10-S10. [Journal article]

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URL: https://www.nature.com/jhh/journal/v31/n10/full/jhh201763a.html

Abstract

Introduction: microRNAs are small, non-coding RNAs which are potentially valuable markers of cardiovascular disease (CVD) risk, including hypertension. This novel investigation aims to profile circulating serum concentrations of microRNAs in premature CVD patients to identify microRNAs that correlate best with hypertension.Methods: Serum samples from an existing cohort of 75 premature CVD patients were analysed for expression of 68 CVD-related microRNAs. Patients had been screened for the methylenetetrahydrofolate reductase (MTHFR) gene polymorphism C677T, a risk factor for hypertension. Samples had been collected at baseline and following intervention with riboflavin, co-factor for the MTHFR enzyme, as part of a placebo-controlled double-blind, randomized trial. The associations between miRNA expression and blood pressure at baseline and post-intervention were investigated. Comparisons of data between CC and TT MTHFR genotype groups, and in response to intervention, were assessed using ANOVA, Pearson’s correlation and corrected t-test statistical analyses. Results: microRNA expression was successfully detected and quantified in all samples. At baseline miR-199a-5p expression was inversely correlated (r=-0.51;p<0.001) with blood pressure in patients with the MTHFR TT genotype only. The decrease in blood pressure in those TT genotype patients who responded to riboflavin intervention was inversely correlated with miR-199a-5p expression (r=-0.55;p<0.05). In vitro and in silico analysis of miR-199a-5p function was also performed.Conclusions: This is the first study to identify miR-199a-5p as a potential serum biomarker of blood pressure in a cohort of at-risk CVD patients. We propose that serum profiling of microRNAs could aid early prediction of CVD and may lead to improved treatment regimes.

Item Type:Journal article
Keywords:mir-199a-5p, Hypertension, MTHFR, cardiovascular disease
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences > School of Nursing
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Genomic Medicine
Institute of Nursing and Health Research
Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Institute of Nursing and Health Research > Managing Chronic Illness Research Centre
Biomedical Sciences Research Institute
ID Code:38818
Deposited By: Dr Declan McKenna
Deposited On:19 Oct 2017 13:43
Last Modified:19 Oct 2017 13:43

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