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Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability

Dosunmu, Yewande and Owusu-Apenten, Richard (2017) Effects of Ascorbic Acid, Dehydroascorbic Acid and Methotrexate on Breast Cancer Cell Viability. Journal of Applied Life Sciences International, 14 (2). [Journal article]

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DOI: 10.9734/JALSI/2017/37185

Abstract

Aims: To examine the effects of ascorbic acid (AA), dehydroascorbic acid (DHA) and methotrexate(MTX) combined treatments on (MDA-MB-231) breast cancer cell viability and intracellular reactive oxygen species (ROS).Study Design: In-vitro method.Place and Duration of Study: Biomedical Sciences Research Institute, University of Ulster, Coleraine, BT52 1SA, United Kingdom. September 2016-2017Methodology: Cytotoxicity tests were performed with MTX (0.01- 1000 µmol/l) alone or in combination with AA or DHA, for 72 h. Cell viability was measured by 3-4,5 dimethylthiazol-2,5 diphenyl tetrazolium bromide (MTT) or Sulforhodamine B (SRB) assays. Intracellular ROS was measured by 2’,7’-dichlorofluroscein diacetate assay.Results: Treatments of MDA-MB231 cells with single agents, showed dose dependent response with 50% inhibition of cell viability (IC50) of 110.5-201.4 µmol/l (MTX), 2237-5703 µmol/l (AA) or 2474 µmol/l (DHA). Combination studies showed clear synergisms for MTX (~10 µmol/l) and DHA or AA (1100 µmol/l) but weak or no interactions at other concentrations. Three days combination treatment of DHA showed decrease of ROS, which was reversed by MTX (>10 µmol/l).Conclusions: Co-treatment of methotrexate with AA or DHA showed synergism (C1<1.0) and enhanced cytotoxicity of the anti-folate towards MDA-MB-231 breast cancer cells. Intracellular ROSdecreased with AA and DHA treatment, which might be useful for reducing MTX-related oxidativestress.

Item Type:Journal article
Keywords:Ascorbic acid; dehydroascorbic acid; methotrexate; breast cancer; MDA-MB-231 cells; Reactive oxygen species. Intracellular oxidative stress
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
ID Code:38933
Deposited By: Dr Richard Owusu-Apenten
Deposited On:10 Nov 2017 15:30
Last Modified:10 Nov 2017 15:30

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