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Genomics and metagenomics of trimethylamine-utilizing Archaea in the human gut microbiome

Borrel, Guillaume, McCann, Angela, Deane, Jennifer, Neto, Marta C, Lynch, Denise B, Brugere, Jean- Francois and O' Toole, Paul W (2017) Genomics and metagenomics of trimethylamine-utilizing Archaea in the human gut microbiome. ISME, 11 . pp. 2059-2074. [Journal article]

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URL: https://www.nature.com/articles/ismej201772

DOI: doi:10.1038/ismej.2017.72

Abstract

The biological significance of Archaea in the human gut microbiota is largely unclear. We recently reported genomic and biochemical analyses of the Methanomassiliicoccales, a novel order of methanogenic Archaea dwelling in soil and the animal digestive tract. We now show that these Methanomassiliicoccales are present in published microbiome data sets from eight countries. They are represented by five Operational Taxonomic Units present in at least four cohorts and phylogenetically distributed into two clades. Genes for utilizing trimethylamine (TMA), a bacterial precursor to an atherosclerogenic human metabolite, were present in four of the six novel Methanomassiliicoccales genomes assembled from ELDERMET metagenomes. In addition to increased microbiota TMA production capacity in long-term residential care subjects, abundance of TMA-utilizing Methanomassiliicoccales correlated positively with bacterial gene count for TMA production and negatively with fecal TMA concentrations. The two large Methanomassiliicoccales clades have opposite correlations with host health status in the ELDERMET cohort and putative distinct genomic signatures for gut adaptation.

Item Type:Journal article
Keywords:Human gut microbiome, archaea, genomics, metagenomics
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Genomic Medicine
Biomedical Sciences Research Institute
ID Code:39193
Deposited By: Dr Jennifer Deane
Deposited On:09 Feb 2018 12:00
Last Modified:09 Feb 2018 14:08

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