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Chitosan/Nitrogen Doped Reduced Graphene Oxide Modified Biosensor for Impedimetric Detection ofmicroRNA

Eksin, E, Bikkarolla, SK, Erdem, A and Papakonstantinou, P (2018) Chitosan/Nitrogen Doped Reduced Graphene Oxide Modified Biosensor for Impedimetric Detection ofmicroRNA. ELECTROANALYSIS, n/a . pp. 1-11. [Journal article]

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URL: http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-4109

DOI: 10.1002/elan.201700663

Abstract

The development of a low-cost and disposable biosensor platform for the sensitive and rapid detection of microRNAs (miRNAs) is of great interest for healthcare, pharmaceuticals, and medical science. We designed an impedimetric biosensing platform using Chitosan (CHIT)/nitrogen doped reduced graphene oxide (NRGO) conductive composite to modify the surface of pencil graphite electrodes (PGE) for the sensitive detection of miRNAs. An initial optimisation protocol involved investigation of the effect of NRGO concentration and miR 660 DNA probe concentration on the response of the modified electrode. After the optimization protocol, the sequence-selective hybridization between miR 660 DNA probe and its RNA target was evaluated by measuring changes on charge transfer resistance, Rct values. Moreover, the selectivity of impedimetric biosensor was tested in the presence of non-complementary miRNA (NC) sequences, such as miR 34a and miR 16. The hybridization process was examined both in phosphate buffer (PBS) and in PBS diluted fetal bovine serum (FBS:PBS) solutions. The biosensor demonstrated a detection limit of 1.72 μg/mL in PBS and 1.65 μg/mL in FBS:PBS diluted solution. Given the easy, quick and disposable attributes, the proposed conductive nanocomposite biosensor platform shows great promise as a low-cost sensor kit for healthcare monitoring, clinical diagnostics, and biomedical devices.

Item Type:Journal article
Keywords:Nitrogen doped reduced graphene oxide · chitosan · microRNA · electrochemical impedance spectroscopy · pencil graphite electrode
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:39424
Deposited By: Professor Pagona Papakonstantinou
Deposited On:06 Feb 2018 09:14
Last Modified:19 Jan 2019 23:23

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