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Antimicrobial effects of freshwater sponge extracts from laboratory grown and riverine sponges

Cartwright, Allison, Arnscheidt, Joerg, Dooley, James and McGonigle, Chris (2018) Antimicrobial effects of freshwater sponge extracts from laboratory grown and riverine sponges. In: SfAM 7th ECS research symposium, Birmingham. SfAM. 2 pp. [Conference contribution]

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Abstract

The project tested whether freshwater sponge extracts had antimicrobial properties, which affect the growth of nosocomial bacteria. Furthermore, it investigated if antimicrobial properties of the extracts originated from sponges or their microbiome. Two freshwater sponge species Spongilla lacustris and Ephydatia fluviatilis were tested for antimicrobial properties. Sponges of each species were collected from rivers (full microbiome) and laboratory grown in sterile water (reduced microbiome). Sponge samples were dried and extracted in methanol. After evaporation of methanol extracts were resuspended in DMSO. Antimicrobial properties were assessed by disc diffusion and recording the minimal inhibitory zone (MIZ) for the bacteria Acinetobacter baumanniii, Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis.None of the tested sponge extracts suppressed the growth of P. aeruginosa, S. aureus or S. epidermidis. Only K. pneumoniae was inhibited by all extracts, with the extracts from laboratory grown sponges resulting in significantly wider MIZs (U=73-89, p=0.004-0.019). A. baumanniii, E. coli and E. faecalis were only inhibited by the extract from riverine S. lacustris. Freshwater sponges are potential sources of antimicrobial compounds, particularly against K. pneumoniae. The antimicrobial properties of laboratory grown sponges suggested that the bioactive molecules which inhibited K. pneumoniae originated from the sponge. However, the enhanced antimicrobial properties of S. lacustris from rivers indicated that there was also a contribution by the microbiome to these extracts’ inhibitory efficacy. Successful clinical therapies against nosocomial bacteria with multidrug resistance will increasingly require new antimicrobial compounds. This study has provided evidence that freshwater sponges contain bioactive molecules. The latter do inhibit bacterial growth and therefore could be a source of antimicrobial compounds, which may originate both from the sponges and their microbiome.

Item Type:Conference contribution (Poster)
Keywords:Porifera, freshwater sponges, antibiotic resistance, antimicrobial resistance
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences > School of Geography and Environmental Sciences
Faculty of Life and Health Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute > Nutrition Innovation Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute
Environmental Sciences Research Institute > Coastal Systems
Environmental Sciences Research Institute
Environmental Sciences Research Institute > Freshwater Sciences
ID Code:39883
Deposited By: Dr Joerg Arnscheidt
Deposited On:07 Apr 2018 09:25
Last Modified:07 Apr 2018 09:25

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